Aptamer-based fiber sensor for thrombin detection

作者：Coelho Luis^*; Marques Martins de Almeida Jose Manuel; Santos Jose Luis; da Silva Jorge Pedro Alberto; Martins Maria Cristina L; Viegas Diana; Queiros Raquel B

来源：Journal of Biomedical Optics, 2016, 21(8): 087005.

DOI：10.1117/1.JBO.21.8.087005

• surface plasmon resonance
• long period fiber gratings
• titanium dioxide
• fiber optic refractometer
• thrombin-binding aptamer

The detection of thrombin based on aptamer binding is studied using two different optical fiber-based configurations: long period gratings coated with a thin layer of titanium dioxide and surface plasmon resonance devices in optical fibers coated with a multilayer of gold and titanium dioxide. These structures are functionalized and the performance to detect thrombin in the range 10 to 100 nM is compared in transmission mode. The sensitivity to the surrounding refractive index (RI) of the plasmonic device is higher than 3100 nmRIU(-1) in the RI range 1.335 to 1.355, a factor of 20 greater than the sensitivity of the coated grating. The detection of 10 nM of thrombin was accomplished with a wavelength shift of 3.5 nm and a resolution of 0.54 nM.

• 出版日期2016-8