Development of a Novel Versatile Method for Determination of two Antihistamines in Association with Naphazoline Using Cathodically Pretreated Boron-doped Diamond Electrode

作者：Oliveira Thiago da Costa; Freitas Jhonys Machado; Abarza Munoz Rodrigo Alejandro; Richter Eduardo Mathias^*

来源：Electroanalysis, 2018, 30(5): 868-876.

DOI：10.1002/elan.201700658

• Simultaneous Analysis
• Environmentally Friendly Method
• Fast Analysis
• Portable System
• Quality Control

Antihistamines such as pheniramine (PHN) or chlorpheniramine (CPH) are commonly associated with naphazoline (NPZ) in eye drops and nasal decongestants. In this work, a batch-injection analysis system with multiple pulse amperometric (BIA-MPA) detection has been applied for the first time for fast simultaneous determination of naphazoline (NPZ) and pheniramine (PHN) or NPZ and chlorpheniramine (CPH). PHN or CPH was selectively detected at +1.1V and both PHN and NPZ or CPH and NPZ were detected at +1.3V using boron doped diamond (BDD) as working electrode and Britton-Robinson (BR) buffer (pH=10.0) as supporting electrolyte. The current of NPZ can then be obtained by subtraction of the currents detected at both potential pulses and applying a correction factor (CF). The proposed method presented good intra-day repeatability (RSD between 0.7 and 3.2% for PHN; 0.7 and 2.1% for CPH; 1.5 and 4.0% for NPZ; n=20), high analytical frequency (>80 injections h(-1)), and limits of detection of 0.64, 0.47 and 0.11molL(-1) for PHN, CPH and NPZ, respectively. The results obtained with the proposed method are in agreement with those obtained by HPLC (95% confidence level).

• 出版日期2018-5