科研之友
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摘要
The mapping of contraction forces developed from cells to their extracellular matrix is crucial to understanding how cells regulate their physiological function to adapt to their living environment and cellular processes. This paper reports a novel cell contractility mapping transducer utilizing moire patterns as a visual and quantitative tool. Coherent light diffracted from two closely placed microfabricated periodic substrates is capable of mapping cell contraction forces via mapping the in-plane displacement on the sample substrate. By integrating cell culture environment and automated Fourier-based fringe analysis, the moire pattern generated through microfabricated periodic substrates enables the mapping of cell contraction force distribution through phase changes encoded in carrier moire fringe patterns. We demonstrated utilizing the transducer to map cardiac myocyte contraction under electric stimulation and vascular smooth muscle cell contractility evolutions triggered by agonist. Given the unique properties of optical moire techniques (i.e., their automatic displacement and strain contouring and their magnification effect for small displacements), this new approach would be an improvement over existing techniques since it can be integrated with the existing engineered substrates and provide a direct contour of cell forces and fast detection of abnormal cell contractions. [2009-0294]
- 出版日期2010-8
