Binding affinities of carbohydrate-conjugated chlorins for galectin-3

作者：Park Young K; Bold Bayarmaa; Cui Bing Cun; Bai Jin Quan; Lee Woo kyoung; Shim Young Key^*

来源：Bulletin of the Korean Chemical Society, 2008, 29(1): 130-134.

• biosensor
• cancer marker
• carbohydrate
• chlorin
• galectins

Carbohydrate-conjugated chlorins were synthesized for use as biosensors for the detection of the galectin-3 cancer marker. We used ELISA, SDS-gel electrophoresis, and Bradford assays to examine the binding of galectins to D-(+)-galactose- and beta-lactose-conjugated chlorins. The binding affinities of these conjugated chlorins for galectin-3 were quantified using fluorescence spectroscopy. The fluorescence emission of the carbohydrate-conjugated chlorins decreased as the amount of galectin-3 in the binding reaction increased over a limited concentration range, indicating that carbohydrate-conjugated chlorins are potentially useful fluorescence biosensors for the galectin-3 cancer marker.

• 出版日期2008-1-20