科研之友
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摘要
This study demonstrated the effect of tailored electrospun scaffolds of poly(epsilon-caprolactone) (PCL) and its blending with water-soluble poly(N-vinyl-2-pyrrolidone) (PVP) on dynamics of adhesion and motility of adipose-derived stem cells. We produced PCL and PCL/PVP electrospun scaffolds with circular hole pattern to create variation of fiber density (low and high density area). Scanning electron microscopy revealed that PCL/PVP scaffolds had nano-scale pores on the surface of fibers (nanoporisity) due to PVP extraction. The nanoporosity of PCL/PVP scaffolds showed the increase in water contact angle and the decrease in cell adhesion and proliferation compared to PCL alone scaffolds. PCL alone scaffolds supported cell attachment, exhibiting large spread area and low circularity during 24 h. In addition, the expressions of vinculin, F-actin and phosphorylation of focal adhesion kinase increased in the cells on PCL scaffolds. In order to determine the effect of PCL scaffold with different fiber density on cell motility, we performed single-cell tracking experiments with time-lapse microscopy. In high fiber density area of PCL scaffolds, cells migrated slowly and directionally in a fiber-independent manner. On the other hand, cells in low fiber density moved fast and fiber-dependently, suggesting that large pore size of scaffolds from low fiber density allow cells to migrate or infiltrate inside the scaffolds. Thus, we demonstrated that the nano/microstructural morphological cues of the scaffolds, e.g. nanoporosity of fibers and fiber density and thus porosity, plays a key role in possible targets to regulate cell behaviors, suggesting that well-tailored electrospun scaffolds with desired composition and fiber density are required for a potential means for application in tissue engineering.
- 出版日期2013-12
