A simple one-step real-time RT-PCR for diagnosis of dengue virus infection

作者：Godoy dos Santos Harryson Wings; Ramos Silva Poloni Telma Regina; Souza Kelly Paula; Menjon Muller Vanessa Danielle; Tremeschin Flavia; Nali Livia Christensen; Fantinatti Leandro Ricardo; Amarilla Alberto Anastacio; Alfonso Castro Helda Liz; Nunes Marcio Roberto; Casseb Samir Mansour; Vasconcelos Pedro Fernando; Badra Soraya Jabur; Moraes Figueiredo Luiz Tadeu; Aquino Victor Hugo^*

来源：Journal of Medical Virology, 2008, 80(8): 1426-1433.

DOI：10.1002/jmv.21203

• dengue
• diagnosis
• real-time RT-PCR

Dengue is the most important arbovirus disease in tropical and sub-tropical countries, and can be caused by infection with any of the four-dengue virus (DENV) serotypes. Infection with DENV can lead to a broad clinical spectrum, ranging from sub-clinical infection or an influenza-like disease known as dengue fever (DF) to a severe, sometimes fatal, disease characterized by hemorrhage and plasma leakage that can lead to shock, known as dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). The diagnosis of dengue is routinely accomplished by serologic assays, such as IgM and IgG ELISAs, as well as HI tests, analyzing serum samples obtained from patients with at least 7 days of symptoms onset. These tests cannot be used for diagnosis during the early symptomatic phase. In addition, antibodies against dengue are broad reactive with other flaviviruses. Therefore, a specific diagnostic method for acute DENV infection is of great interest. In that sense, the real-time RT-PCR has become an important tool that can be used for early and specific detection of dengue virus genome in human serum samples. This study describes a simple, specific, and sensitive real-time RT-PCR for early diagnosis of dengue virus infection.

• 出版日期2008-8