Pioneering Axons Regulate Neuronal Polarization in the Developing Cerebral Cortex

作者：Namba Takashi; Kibe Yuji; Funahashi Yasuhiro; Nakamuta Shinichi; Takano Tetsuya; Ueno Takuji; Shimada Akiko; Kozawa Sachi; Okamoto Mayumi; Shimoda Yasushi; Oda Kanako; Wada Yoshino; Masuda Tomoyuki; Sakakibara Akira; Igarashi Michihiro; Miyata Takaki; Faivre Sarrailh Catherine; Takeuchi Kosei; Kaibuchi Kozo^*

来源：Neuron, 2014, 81(4): 814-829.

DOI：10.1016/j.neuron.2013.12.015

The polarization of neurons, which mainly includes the differentiation of axons and dendrites, is regulated by cell-autonomous and non-cell-autonomous factors. In the developing central nervous system, neuronal development occurs in a heterogeneous environment that also comprises extracellular matrices, radial glial cells, and neurons. Although many cell-autonomous factors that affect neuronal polarization have been identified, the microenvironmental cues involved in neuronal polarization remain largely unknown. Here, we show that neuronal polarization occurs in a microenvironment in the lower intermediate zone, where the cell adhesion molecule transient axonal glycoprotein-1 (TAG-1) is expressed in cortical efferent axons. The immature neurites of multipolar cells closely contact TAG-1-positive axons and generate axons. Inhibition of TAG-1-mediated cell-to-cell interaction or its downstream kinase Lyn impairs neuronal polarization. These results show that the TAG-1-mediated cell-to-cell interaction between the unpolarized multipolar cells and the pioneering axons regulates the polarization of multipolar cells partly through Lyn kinase and Rac1.

• 出版日期2014-2-19