Antioxidant effect of erdosteine and lipoic acid in ovarian ischemia-reperfusion injury

作者：Dokuyucu R^*; Karateke A; Gokce H; Kurt R K; Ozcan O; Ozturk S; Tas Z A; Karateke F; Duru M

来源：European Journal of Obstetrics & Gynecology and Reproductive Biology, 2014, 183: 23-27.

DOI：10.1016/j.ejogrb.2014.10.018

• Ischaemia/reperfusion
• Ovarian torsion
• Erdostein
• Alpha lipoic acid
• Follicular heritage

Objective: To investigate the effects of erdosteine and alpha lipoic acid (ALA) in a rat model of ovarian ischaemia-reperfusion injury.
Study design: Forty-eight female Wistar albino rats were separated, at random, into six groups of eight rats. The groups were classified as: sham, torsion, detorsion, detorsion + erdosteine 100 mg/kg, detorsion + alpha lipoic acid (ALA) 100 mg/kg, and detorsion + erdosteine + ALA. The investigators executing the biochemical and histological analyses were blinded to the randomization until the end of the study.
Results: The TOS (Total Oxidant Status) and OSI (Oxidative Stress Index) levels are higher in the Torsion and Detorsion groups when compared with the ones in the Sham group (p < 0.05). Strong correlation was found between OSI and total histological score in the sham, torsion and detorsion groups (r = 0.765, p < 0.001). The mean levels of TOS and OSI in the rats that received erdosteine and/or ALA were significantly lower compared with the sham, torsion and detorsion groups (p < 0.05). Mean TOS and mean OSI were lower in the detorsion + erdosteine + ALA group compared with the detorsion + erdosteine and detorsion + ALA groups (p < 0.05). In comparison with the detorsion group, the numbers of primordial follicles (p = 0.006) and primary follicles (p = 0.036) were increased in the groups that received erdosteine and/or ALA.
Conclusions: Erdosteine and ALA decreased ischaemia-reperfusion injury in an experimental rat ovarian torsion model; combination treatment had a greater effect than either agent alone. Treatment with erdosteine and/or ALA was found to preserve the loss of reproductive capacity normally observed after ovarian torsion.

• 出版日期2014-12