Determination of glycated hemoglobin on the basis of spectral shifting from protein-dye interaction

作者：Kim Eun Joong; Song Seung Yeon; Lee Bun Yeoul; Yoon Hyun C^*

来源：BioChip Journal, 2010, 4(1): 16-21.

DOI：10.1007/s13206-010-4103-2

• Glycated hemoglobin
• Glycated protein
• Hemoglobin A(1c)
• Optical sensing
• Spectral shifting

A method for the detection of glycated proteins was developed on the basis of spectral shifting observed for 1-(4-Boronophenylazo)-2-hydroxy-3,6-naphthalene-disulfonic acid (azo-BA) dye. Hemoglobin A(1c), a type of glycated hemoglobin, is an important indicator of diabetes mellitus control and management. We have employed the azo-BA dye containing a boronic acid group linked to an aromatic chromophore. The boronic acid group of the dye specifically binds to the carbohydrate chain of HbA(1c) via cis-diol interaction, and its spectra exhibit a unique chromatic redshift. Because the reaction is spontaneous at neutral pH, this method provides an advantage of short detection time. After the azo-BA dye/HbA(1c) reaction commenced, the concentration of conjugate was measured by registering spectral shifts. The measurements revealed that the method has a dynamic detection range of 3-15% (HbA(1c)/total hemoglobin), which covers the required clinical reference range. This method has shown reproducible results; the coefficient of variation of the test was 3.60%. Thus, the proposed method was found suitable for measuring %HbA(1c) and would be applicable for diabetes mellitus control.

• 出版日期2010-3-20