Application of high-speed counter-current chromatography for preparative separation of cyclic peptides from Vaccaria segetalis

作者：Wang, Xiao; Dong, Hongjing; Liu, Yuqin; Yang, Bin; Wang, Xia; Huang, Luqi^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2011, 879(11-12): 811-814.

DOI：10.1016/j.jchromb.2011.02.001

• Vaccaria segetalis
• HSCCC
• Preparative separation
• Segetalin B
• Segetalin A

Following an initial clean-up step on silica gel, high-speed counter-current chromatography (HSCCC) was used to separate cyclic peptides from an extract of the seeds of Vaccaria segetalis. The two-phase solvent system used for HSCCC separation was composed of petroleum ether-ethyl acetate-methanol-water at an optimized volume ratio of 0.5:3.5:1:5. From 190 mg of crude extract, 38.0 mg of segetalin B and 28.5 mg of segetalin A were obtained with purities of 98.1% and 95.6% as determined by HPLC, respectively. The chemical structures of the target compounds were confirmed by high resolution electrospray ionization time of flight MS (HRESI-TOF-MS) and (1)H NMR analyses.

• 出版日期2011-4-1
• 单位山东省科学院; 中国中医科学院