Differential responses to isoprenoid, N-6-substituted aromatic cytokinins and indole-3-butyric acid in direct plant regeneration of Eriocephalus africanus

作者：Madzikane Mlungwana Olwethu; Moyo Mack; Aremu Adeyemi O; Plihalova Lucie; Dolezal Karel; Van Staden Johannes^*; Finnie Jeffrey F

来源：Plant Growth Regulation, 2017, 82(1): 103-110.

DOI：10.1007/s10725-016-0242-3

• Auxins
• Cytokinins
• Flavonoids
• Plant regeneration
• Phenolics

Eriocephalus africanus is a medicinal and aromatic plant species that is part of South Africa's remarkable diversity. As a result of illegal and over-harvesting, most plant communities have become unsustainable and as such, effective and efficient conservation strategies have to be implemented. In the present study, an isoprenoid cytokinin (CK): isopentenyladenine (iP) and four aromatic CKs namely benzyladenine (BA), meta-topolin (mT), meta-topolin riboside (mTR) and 6-(3-hydroxybenzylamino)-9-(tetrahydropyran-2-yl)purine (mTTHP) at 1, 5 or 10 mu M were evaluated for in vitro plant regeneration in E. africanus. Different concentrations of indole-3-butyric acid (IBA) were also evaluated for shoot and root organogenesis. The highest number of shoots was produced by mT (1 and 5 mu M) treatment, longest shoots were stimulated by iP (1 mu M) and the highest fresh mass was obtained in BA (5 and 10 mu M), mT (5 and 10 mu M) and mTTHP (5 mu M)-treated plants. During acclimatization, all the in vitro plants obtained from the lowest concentration of CKs survived and 1 mu M iP regenerants recorded a 100% survival rate. For the rooting experiment, more vigorous adventitious roots were observed in the 1 mu M IBA treatment. All IBA treatments had 100% survival rate after 6 weeks of acclimatization. Overall, the concentration and type of plant growth regulators had a remarkable influence on the growth and development of in vitro-derived E. africanus.

• 出版日期2017-5