A sensitive electrochemical DNA biosensor for antineoplastic drug 5-fluorouracil based on glassy carbon electrode modified with poly (bromocresol purple)

作者：Zeybek Derya Koyuncu^*; Demir Burcu; Zeybek Bulent; Pekyardimci Sule

来源：Talanta, 2015, 144: 793-800.

DOI：10.1016/j.talanta.2015.06.077

• Poly(bromocresol purple)
• 5-fluorouracil
• DNA biosensor
• DNA-drug interaction
• intercalation

This paper describes an electrochemical sensor for the first time based on poly(bromocresol purple) (P (BCP)) developed to observe the interaction between 5-fluorouracil (5-FU) and fish sperm double strand DNA (dsDNA). The P(BCP) film was electrosynthesized by cyclic voltammetry method on the glassy carbon electrode (GCE). The dsDNA was electrochemically immobilized on the surface of P(BCP) modified GCE and the DNA biosensor was prepared. The interaction mechanism of dsDNA with 5-FU was investigated by differential pulse voltammetry using this biosensor. A decrease in the guanine oxidation peak current of the biosensor was observed after the interaction of dsDNA and 5-FU in 0.5 mol L-1 acetate buffer (pH 4.8) containing 0.02 mol L-1 NaCl. The accumulation time and dsDNA concentration were optimized to obtain the best peak current response. Under optimum conditions, the linear response on the guanine signal decreasing curve was observed in the 5-FU concentration range of 1.0-50 mg L-1. The interaction mechanism between dsDNA and 5-FU was further investigated by UV-vis spectroscopy and viscometer. The results reveal that intercalation is the primary mode of interaction between 5-FU and dsDNA.

• 出版日期2015-11-1