科研之友
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摘要
Introduction: the human androgen receptor (AR) gene possesses two trinucleotide polymorphic repeats, (CAG and GGN) that affect the amount of AR protein translated. In this study, we genotyped these polymorphic tracts in a representative sample of Caucasian children (Tanner <= 5), 152 boys (11.5 +/- 2.6 yrs) and 116 girls (10.1 +/- 3.2 yrs) from Spain and investigated their association with bone mass.
Methods: the length of CAG and GGN repeats was determined by PCR and fragment analysis. Body composition was assessed by dual energy x-ray absorptiometry (DXA). Individuals were grouped as CAG short (CAG(S)) if harboring repeat lengths of <= 21 and CAG long (CAG(L)) if CAG >21. Moreover, subjects were grouped as GGN short (GGN(S)) if harboring repeat lengths of <= 23 and GGN long (GGN(L)) if GGN >23.
Results: in boys, significant differences in height, body mass, whole body bone mineral density (BMD) and content (BMC), upper extremities BMC, lower extremities BMC, femoral neck BMD, Ward's triangle BMC and BMD and lumbar spine BMD were observed between CAG(S) and CAG(L) groups (P<0.05). Thus, upper extremities BMD differed between GGN(S) and GGN(L) groups. After adjusting for confounding variables, only upper extremities BMD between GGN(S) and GGN(L) groups remained significant (P<0.05). No differences were observed in girls in any measured site in relation to either CAG or GGN polymorphisms length.
Conclusions: our results support the hypothesis that longer alleles of the AR CAG and GGN polymorphisms are associated with increased bone mass in prepubertal boys.
- 出版日期2015-12
