Tumor necrosis factor (TNF)-alpha cupregulates progesterone receptor-A by activating the NF-kappa B signaling pathway in human decidua after labor onset

作者：Jiang Z Y; Guo Y Y; Ren H B; Zou Y F; Fan M S; Lv Y; Han P; De W; Sun L Z^*

来源：Placenta, 2012, 33(1): 1-7.

DOI：10.1016/j.placenta.2011.09.004

• Decidua
• TNF-alpha
• PR-A/PR-B ratio
• NF-kappa B
• COX-2

To date, the relationship between inflammatory cytokines and progesterone receptors (PRs) has been little studied, although both mediate the mechanism of parturition in human deciduas. Thus, the aim of study was to investigate the role of an inflammatory cytokine, tumor necrosis factor (TNF)-alpha, in regulating progesterone withdrawal in decidua at human parturition. TNF-alpha levels and PR isoforms were compared in intrauterine deciduas from women who were in labor (IL, n = 10) or who were not in labor (NIL, n = 10). Nuclear factor-kappaB (NF-kappa B) signaling and PR status were analyzed in NIL deciduas after TNF-alpha stimulation. lmmunohistochemistry, western blotting, ELISA and reverse transcription-polymerase chain reaction (RT-PCR) were used to localize and quantitate protein and mRNA expression. TNF-alpha immunostaining, protein levels, PR-A/PR-B ratio and COX-2 level were significantly higher in IL deciduas (all P < 0.05). NF-kappa B was activated by TNF-alpha after 24 h stimulation in a dose-dependent manner, and was significantly inactivated by the NF-kappa B inhibitor panepoxydone, which was associated with decreased PR-A and COX-2 expression (P < 0.05) in not in labor deciduas. In conclusion, TNF-alpha may have an important role in regulating progesterone withdrawal in human decidua following labor onset.

• 出版日期2012-1
• 单位南京医科大学