Distinctive expression of interleukin-23 receptor subunits on human Th17 and gamma delta T cells

作者：Wines Bruce D; Yap May L; Powell Maree S; Tan Peck Szee; Ko K Kerry; Orlowski Eva; Hogarth P Mark

来源：Immunology and Cell Biology, 2017, 95(3): 272-279.

DOI：10.1038/icb.2016.93

The interleukin-23 (IL-23) pathway, T helper 17 (Th17) cells and gamma delta T cells, which respond to IL-23, have major pro-inflammatory roles. We have used unique IL-23 receptor ( IL-23R) subunit-specific monoclonal antibodies, X67 and X68, and IL-12 receptor beta-1 subunit ( IL-12R beta 1) expression levels to evaluate the IL-23R complex on CD4 alpha beta TCR Th17 cells and on gamma delta T cells. Both IL-23R and IL-12Ra1 subunits constitute the functional IL-23R. Expression of the IL-23R subunit by cultured Th17 cells was heterogeneous. Th17 cells expressed consistent high levels of the IL-12R beta 1 subunit, which appeared a better predictor of responsiveness to IL-23 than the expression of the IL-23R subunit. Moreover, sorting memory CD4 T cells by high IL-12Ra1 expression selectively enriched cells committed to IL-17 production from the blood. IL-23R expression was also observed on freshly isolated and cultured gamma delta T cells and the cultured gamma delta T cells were not responsive to IL-23.

• 出版日期2017-3