A Rapid High Performance Liquid Chromatographic Method for Determination of Tiamulin Residue in Swine Tissues

作者：Cheng Lin Li^*; Zhang Su Xia; Shen Jian Zhong; Wang Zhan Hui; Li Yan Shen; Feng Pei Sheng

来源：Chinese Journal of Analytical Chemistry, 2009, 37(5): 718-720.

• Tiamulin
• pork
• swine liver
• high performance liquid chromatography

A rapid method has been developed for the determination of tiamulin (TML) residue in swine tissues by high performance liquid chromatography. The samples were extracted with phosphate buffer-ethyl acetate solution, and then centrifuged by 5000 rpm for 10 min, 0.1% fartrate solution was added in the supernatant obtained with liguid-liquid-liquid extraction, and the organic phase was eveporated. After fats having been removed with hexan, further purification was performed with Oasis HLB solid phase extraction column. The TML residue was determined by HPLC with a mobile phase of acetonitrile-0.0125% acetic acid(42:58, V/V) and UV detection at 210 nm. The average recovery rates of TML in pork and swine liver were in the range of 80.1% - 92.7% when spiked 25 - 500 mu g/kg. The RSD ranged from 2.0% to 13.9%. The detection limits were 5 mu g/kg and 8 mu g/kg for pork and swine liver, respectively.

• 出版日期2009-5
• 单位中国农业大学