Depletion of florfenicol and florfenicol amine residues in chicken eggs

作者：Filazi A^*; Sireli U T; Yurdakok B; Aydin F G; Kucukosmanoglu A G

来源：British Poultry Science, 2014, 55(4): 460-465.

DOI：10.1080/00071668.2014.935701

1. The aim of this study was to develop a suitable method for the analysis of florfenicol (FF) and its metabolite florfenicol amine (FFA) in chicken eggs and to determine FF and FFA residue depletion in eggs of laying hens.
2. The analytes were extracted from yolk, albumen and whole egg by phosphate buffer and ethyl acetate. Following purification, samples were analysed by high-performance liquid chromatography.
3. Fifty laying hens were divided into 5 groups, and each hen received doses of 20mg/kg FF: Group 1 (received a single oral dose by gavage); Group 2 (a single intramuscular dose); Group 3 (a single subcutaneous dose); Group 4 (multiple oral doses for 3d) and Group 5 (multiple oral doses for 5d).
4. Limits of detection and of quantitation values were 1.94 and 6.45g/10(9)g (ppb) for FF, respectively, and 0.48 and 1.58ppb for FFA, respectively. Relative standard deviation values of intra-day and inter-day variation below 11% also confirmed the usefulness of the method for analysing FF and FFA in eggs.
5. From the first day of both oral and parenteral administration, FF and FFA were detected at 0.1% and 0.08% of dosage, respectively, and 57% of the drugs were eliminated from the egg yolk. Elimination time of FF was 8d in Groups 1, 2 and 3; 9d in Group 4 and 10d in Group 5.

• 出版日期2014-7-4