Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin: II. Introduction of an O-H center dot center dot center dot S gamma-Fe hydrogen bond increased the reduction potential by 65 mV

作者：Boenisch Heiko; Schmidt Christian L; Bianco Pierre; Ladenstein Rudolf

来源：Journal of Biological Inorganic Chemistry, 2007, 12(8): 1163-1171.

DOI：10.1007/s00775-007-0289-8

• pyrococcus abyssi
• rubredoxin
• reduction potential
• ultrahigh-resolution X-ray crystallography

The effect of D-H center dot center dot center dot S gamma-Fe hydrogen bonding on the reduction potential of rubredoxin was investigated by the introduction of an O-H center dot center dot center dot S gamma-Fe hydrogen bond on the surface of Pyrococcus abyssi rubredoxin. The formation of a weak hydrogen bond between Ser44-O gamma and Cys42-S gamma in mutant W4L/R5S/A44S increased the reduction potential by 56 mV. When side effects of the mutation were taken into account, the contribution of the additional cluster hydrogen bond to the reduction potential was estimated to be + 65 mV. The structural analysis was based on ultrahigh-resolution structures of oxidized P. abyssi rubredoxin W4L/R5S and W4L/R5S/A44S refined to 0.69 and 0.86 angstrom, respectively.

• 出版日期2007-11