Antimicrobial, Gastroprotective and Healing Effect of the Hydroalcoholic Extract of Astronium fraxinifolium

作者：Pereira Bezerra Martins Anita Oliveira Brito; Albuquerque Siebra Ana Luiza; de Morais Oliveira Cicera Datiane^*; Leite Gerlania Oliveira; Melo Coutinho Henrique Douglas; Athayde Margareth Linde; Bolignon Aline Augusti; Andrade Jacqueline Cosmo; Oliveira Larissa Rolim; Kerntopf Marta Regina; Barbosa Roseli; Alves Santana Cesario Francisco Rafael; Coutinho Thales Silva; Quintans Junior Lucindo Jose; Alencar de Menezes Irwin Rose

来源：Letters in Drug Design and Discovery, 2018, 15(3): 325-334.

DOI：10.2174/1570180814666170213160114

• Astronium fraxinifolium
• Antioxidant
• Modulatory effect
• antifungic effect
• antibacterial activity
• gastroprotection
• healing

Introduction: Astronium fraxinifolium (Anarcadiaceae) is used in folk medicine in inflammation diseases. This study evaluates the chemical profile as well as antioxidant, antimicrobial, gastroprotective and wound healing activities of the hydroalcoholic extract of Astronium fraxinifolium bark (HAAF).
Material and Methods: Phytochemical screening and high-performance liquid chromatography (HPLC). The antioxidant activity was determined by DPPH (2,2-diphenyl, 1-picrihidrazila) and FRAP (ferric fraction reductive) methods. The gastroprotective activity was evaluated against HAAF to classical models of acute gastric: absolute ethanol, acidified ethanol and Indomethacin (50, 100 and 200mg/kg/orally) and physical barrier mechanism (50mg/kg/orally, intraperitoneally), used Swiss mice (Mus musculus). The healing activity of HAAF was identified from tissue shrinkage rate in the control. The antimicrobial activity was observed from the Minimum Inhibitory Concentration method (MIC) and modulation associated with antibiotics (amikacin, gentamicin, benzylpenicilin, imipinem + cilastatin sodium, cephalothin, oxalacina and ampicillin) and anti-fungals (amphotericin B, nystatin, mebendazole and benzoilmetronidazol).
Results: Phytochemical prospection of Chromatography (HPLC) quantified gallic acid (4.25 %), chlorogenic acid (2.11%), caffeic acid (0.73%), rutin (0.08 %) and quercetin (0.29%). Measurement of total flavonoids (0.034 g of quercetin) and phenols (0.538 g of gallic acid) gram of extract showed significant amount. HAAF showed significant antioxidant potential in DPPH and FRAP assays. The MIC did not show clinically relevant activity (>= 1024 mu g/mL). HAAF displayed gastroprotective different doses (50, 100 and 200 mg/Kg) and Healing activity (100 mg/g).
Conclusion: The results obtained in this study provide biological evidence of the antioxidant potential, gastroprotective and wound healing effect of the species.

• 出版日期2018