MRD detection of leukemia relapse using HLA typing by FACS in combination with FISH after mismatched allogeneic stem cell transplantation

作者：Miyachi Mitsuru; Watanabe Eri; Watanabe Nobukazu; Tsuma Yusuke; Kawashima Goto Sachiko; Tamura Shinichi; Imamura Toshihiko^*; Ishida Hiroyuki; Hosoi Hajime

来源：Pediatric Transplantation, 2014, 18(5): E180-E184.

DOI：10.1111/petr.12267

• HLA-Flow
• minimal residual disease
• mismatched HLA allele
• mismatched allo-SCT

Loss of mismatched HLA is a cause of relapse following HLA-mismatched allo-SCT. We directly detected the loss of mismatched HLA alleles in relapsed leukemic cells at a MRD level using HLA typing by multicolor FACS (HLA-Flow) in combination with FISH in the BM of two patients with MLL-AF9-positive AML, at 6 and 10 months after mismatched allo-SCT. HLA-Flow with FISH analysis detected relapsed leukemic cells not expressing a mismatched HLA allele and harboring the MLL rearrangement. Simultaneously, real-time quantitative RT-PCR detected a low copy number of MLL-AF9 transcripts, consistent with MRD detection. HLA-Flow with FISH is a powerful method for detecting molecular relapse after mismatched allo-SCT and provides important information on the HLA expression status of the relapsed leukemic cells to help determine the next intervention.

• 出版日期2014-8
• 单位河北医科大学