A Photocleavable Rapamycin Conjugate for Spatiotemporal Control of Small GTPase Activity

作者：Umeda Nobuhiro; Ueno Tasuku; Pohlmeyer Christopher; Nagano Tetsuo^*; Inoue Takanari

来源：Journal of the American Chemical Society, 2011, 133(1): 12-14.

DOI：10.1021/ja108258d

We developed a novel method to spatiotemporally control the activity of signaling molecules. A newly synthesized photocaged rapamycin derivative induced rapid dimerization of FKBP (FK-506 binding protein) and FRB (FKBP-rapamycin binding protein) upon UV irradiation. With this system and the spatially confined UV irradiation, we achieved subcellularly localized activation of Rac, a member of small GTPases. Our technique offers a powerful approach to studies of dynamic intracellular signaling events.

• 出版日期2011-1-12