A colonisation-inhibition culture consisting of Salmonella Enteritidis and Typhimurium Delta hilAssrAfliG strains protects against infection by strains of both serotypes in broilers

作者：De Cort W; Mot D; Haesebrouck F; Ducatelle R; Van Immerseel F^*

来源：Vaccine, 2014, 32(36): 4633-4638.

DOI：10.1016/j.vaccine.2014.06.077

• Salmonella
• Poultry
• Colonisation-inhibition

Consumption of contaminated poultry meat is still an important cause of Salmonella infections in humans and there is a need for control methods that protect broilers from day-of-hatch until slaughter age against infection with Salmonella. Colonisation-inhibition, a concept in which a live Salmonella strain is orally administered to day-old chickens and protects against subsequent challenge, can potentially be used as control method. In this study, the efficacy of a Salmonella Typhimurium Delta hilAssrAfliG strain as a colonisation-inhibition strain for protection of broilers against Salmonella Typhimurium was evaluated. Administration of a Salmonella Typhimurium Delta hilAssrAfliG strain to day-old broiler chickens decreased faecal shedding and strongly reduced caecal and internal organ colonisation of a Salmonella Typhimurium challenge strain administered one day later using a seeder bird model. In addition, it was verified whether a colonisation-inhibition culture could be developed that protects against both Salmonella Enteritidis and Typhimurium. Therefore, the Salmonella Typhimurium Delta hilAssrAfliG strain was orally administered simultaneously with a Salmonella Enteritidis Delta hilAssrAfliG strain to day-old broiler chickens, which resulted in a decreased caecal and internal organ colonisation for both a Salmonella Enteritidis and a Salmonella Typhimurium challenge strain short after hatching, using a seeder bird model. The combined culture was not protective against Salmonella Paratyphi B varietas Java challenge, indicating serotypespecific protection mechanisms. The data suggest that colonisation-inhibition can potentially be used as a versatile control method to protect poultry against several Salmonella serotypes.

• 出版日期2014-8-6