Distribution of the LDL receptor within clathrin-coated pits and caveolae in rat and human liver

作者：Ivaturi Soumya; Wooten Catherine J; Nguyen Maikhanh D; Ness Gene C; Lopez Dayami^*

来源：Biochemical and Biophysical Research Communications, 2014, 445(2): 422-427.

DOI：10.1016/j.bbrc.2014.02.019

• LDL receptor
• Clathrin
• Caveolin-1
• Rat liver
• Human liver
• Human hepatic cells

Several findings suggest that the low-density lipoprotein (LDL) receptor may internalize different lipoprotein particles via diverse pathways. Using a combination of discontinuous sucrose gradients and Triton solubilization studies, we demonstrated that the LDL receptor could be located simultaneously in clathrin-coated pits and caveolae in rat and human liver and in human hepatocyte-like C3A cells. Treatment with the cholesterol biosynthesis inhibitor, zaragozic acid A, shifted the distribution of the LDL receptor to clathrin containing fractions, whereas treatment with cholesterol or LDL shifted the receptor distribution towards caveolin-1 containing fractions. The LDL-dependent shift of the LDL receptor to caveolae coincided with a reduction in internalization of Bodipy-LDL. Redistribution within plasma membrane microdomains in response to specific treatments resulting in changes in LDL receptor function represents a novel paradigm that could be exploited in the development of a new class of therapeutic drugs.

• 出版日期2014-3-7