Anti-human hepatoma Hep-G2 proliferative, apoptotic, and antimutagenic activity of tagitinin C from Tithonia diversifolia leaves

作者：Liao May Hua; Tsai Yuan Nuan; Yang Chi Yu; Juang Chi Long; Lee Ming Yi; Chang Li Hsueh; Wen Hsiao Chuan^*

来源：Journal of Natural Medicines, 2013, 67(1): 98-106.

DOI：10.1007/s11418-012-0652-0

• Tithonia diversifolia methanolic extract (TDM)
• Tagitinin C
• Hep-G2
• Hepatocellular carcinoma
• Apoptosis
• Xenografts

Tagitinin C, a major sesquiterpenoid, was isolated from the leaves of Tithonia diversifolia. The high morbidity and mortality rate of hepatoma in Taiwan motivated our interest in the investigation of tagitinin C's mechanism against the human hepatocellular carcinoma. The methanolic extract of leaves of T. diversifolia (TDM) and tagitinin C were found to have cytotoxic activities against human hepatoma Hep-G2 cells in the MTT assay with IC50 values of 40.0 +/- A 2.0 and 2.0 +/- A 0.1 mu g/mL, respectively. This compound induced population increase in the sub-G(1) phase and S phase arrest. Treatment with tagitinin C isolated from TDM resulted in activation of both caspase 3 and caspase 8 which suggested that the antiproliferative effect of this compound was caspase-dependent apoptosis. Magnetic resonance techniques indicated that the tumorigenisity of xenografts derived from Hep-G2 cells was retarded by the delivery of tagitinin C (15 mu g/mouse/day) relative to the control counterparts.

• 出版日期2013-1