Molecular mapping and candidate gene analysis for fruit epidermal structure in cucumber

作者：Zhang, Song; Miao, Han; Song, Zichao; Liu, Panna; Wang, Ye; Wehner, Todd C.; Gu, Xingfang^*; Zhang, Shengping^*

来源：Plant Breeding, 2017, 136(5): 767-774.

DOI：10.1111/pbr.12510

• Cucumis sativus L
• fruit epidermal structure
• gene mapping
• molecular marker
• palisade epidermis

Firmness of skin is an important quality of processing cucumbers, which is a feature of the palisade epidermis in these types compared to flat epidermis in fresh cucumbers. This study was conducted to map the Pe (palisade epidermis) and analyse the candidate gene. Populations derived from the cross of two inbred lines, NCG122 with fruit flat epidermis and NCG121 with fruit palisade epidermis, were used to identify the inheritance of palisade epidermis and to map the gene involved in its development. The results showed that the palisade epidermis trait is controlled by a single gene, Pe, that is dominant over flat epidermis. Seven simple sequence repeat (SSR) and five insertion deletion (Indel) markers were identified to be linked to the Pe gene. It was mapped to cucumber chromosome 5 (Chr.5) between SSR14611 and Indelpe12 with genetic distances of 0.3cM and 0.2cM, respectively. The physical distance of the genomic region harbouring the gene was 227.5kb with 26 predicted candidate genes. The accuracy of marker-assisted selection using the molecular markers, Indelpe12 and SSR14611, was 68% and 88%, respectively.

• 出版日期2017-10
• 单位中国农业科学院