Adherent cells in granulocyte-macrophage colony-stimulating factor-induced bone marrow-derived dendritic cell culture system are qualified dendritic cells

作者：Li, Gong Bo; Lu, Guang Xiu^*

来源：Cellular Immunology, 2010, 264(1): 4-6.

DOI：10.1016/j.cellimm.2010.05.009

• Dendritic cell
• Adherent cell
• Bone marrow

A widely-used method for generating dendritic cell (DC) is to culture bone marrow cells in granulocyte-macrophage colony-stimulating factor (GM-CSF)-containing medium for 6-10 days. Usually, non-adherent cells are used as qualified dendritic cells while the adherent ones are discarded as "non-dendritic cells" or macrophages. In this study, we show that the adherent cells are nearly identical to the non-adherent cells in both dendritic cell surface markers expression and main dendritic cell-related functions, hence to prove that these "junk cells" are actually qualified dendritic cells.

• 出版日期2010
• 单位中南大学