Association of CD36 expression and polymorphism with serum biochemical indices in Cherry Valley duck

作者：Wang, D. D.; Li, W. G.; Zhang, Y. Y.^*

来源：Genetics and Molecular Research, 2016, 15(2): 15027994.

DOI：10.4238/gmr.15027994

• CD36
• Gene expression
• Genetic variation
• Serum biochemical indices

Cluster of differentiation 36 (CD36) plays a crucial role in lipid sensing, innate immunity, atherogenesis, and glycolipid metabolism. This aims of this study were to delineate the CD36 mRNA expression profile in 16 duck tissues using relative quantitative realtime PCR and to screen single nucleotide polymorphisms (SNPs) in the duck CD36 gene by PCR-single strand conformation polymorphism and DNA direct sequencing. In addition, this study investigated CD36 gene expression, genetic variation, and their effect on serum biochemical indices in duck. The results showed that CD36 mRNA was expressed in all tissues, and was highly specific to the pituitary and large intestine, and to subcutaneous and abdominal fat. Furthermore, three genotypes of the SNP g.476593 T > C in exon 9 of the duck CD36 gene were identified: MM, MN, and NN. The dominant genotype and allele were MM and M, with frequencies of 0.453 and 0.643, respectively. The genotype distributions deviated from Hardy-Weinberg equilibrium (P < 0.05) and achieved moderate levels of polymorphism in ducks. Correlation results

• 出版日期2016
• 单位贵州大学