A Plasmodium falciparum S33 proline aminopeptidase is associated with changes in erythrocyte deformability

作者：da Silva Fabio L; Dixon Matthew W A; Stack Colin M; Teuscher Franka; Taran Elena; Jones Malcolm K; Lovas Erica; Tilley Leann; Brown Christopher L; Trenholme Katharine R; Dalton John P; Gardiner Donald L; Skinner Adams Tina S^*

来源：Experimental Parasitology, 2016, 169: 13-21.

DOI：10.1016/j.exppara.2016.06.013

• Prolyl aminopeptidase
• Malaria
• Plasmodium falciparum
• Erythrocyte deformability
• Cytoadherence

Infection with the apicomplexan parasite Plasmodium falciparum is a major cause of morbidity and mortality worldwide. One of the Striking features of this parasite is its ability to remodel and decrease the deformability of host red blood cells, a process that contributes to disease. To further understand the virulence of Pf we investigated the biochemistry and function of a putative Pf S33 proline aminopeptidase (PJPAP). Unlike other P. falciparum aminopeptidases, PJPAP contains a predicted protein export element that is non-syntenic with other human infecting Plasmodium species. Characterization of PJPAP demonstrated that it is exported into the host red blood cell and that it is a prolyl aminopeptidase with a preference for N-terminal proline substrates. In addition genetic deletion of this exopeptidase was shown to lead to an increase in the deformability of parasite-infected red cells and in reduced adherence to the endothelial cell receptor CD36 under flow conditions. Our studies suggest that PJPAP plays a role in the rigidification and adhesion of infected red blood cells to endothelial surface receptors, a role that may make this protein a novel target for anti-disease interventions strategies.

• 出版日期2016-10
• 单位McGill