Modifications of excitatory and inhibitory transmission in rat hippocampal pyramidal neurons by acute lithium treatment

作者：Wakita Masahito; Nagami Hideaki; Takase Yuko; Nakanishi Ryoji; Kotani Naoki; Akaike Norio^*

来源：Brain Research Bulletin, 2015, 117: 39-44.

DOI：10.1016/j.brainresbull.2015.07.009

• Acute
• LiCl
• eEPSC
• eIPSC
• Hippocampus

The acute effects of high-dose Li+ treatment on glutamatergic and GABAergic transmissions were studied in the "synaptic bouton" preparation of isolated rat hippocampal pyramidal neurons by using focal electrical stimulation. Both action potential-dependent glutamatergic excitatory and GABAergic inhibitory postsynaptic currents (eEPSC and eIPSC, respectively) were dose-dependently inhibited in the external media containing 30-150 mM Li+, but the sensitivity for Li+ was greater tendency for eEPSCs than for eIPSCs. When the effects of Li+ on glutamate or GABAA receptor-mediated whole-cell responses (I-Glu and I-GABA) elicited by an exogenous application of glutamate or GABA were examined in the postsynaptic soma membrane of CA3 neurons, Li+ slightly inhibited both I-Glu and IGABA at the 150 mM Li+ concentration. Present results suggest that acute treatment with high concentrations of Li+ acts preferentially on presynaptic terminals, and that the Li+-induced inhibition may be greater for excitatory than for inhibitory transmission.

• 出版日期2015-8