A Rapid and Simple Method for the Determination of Acacetin in Rat Plasma by UPLC

作者：Hu Yuanyuan; Zhu Danyan; Wang Chan; Dong Yaoyao; Lian Qingquan^*

来源：Latin American Journal of Pharmacy, 2015, 34(10): 1947-1952.

• acacetin
• pharmacokinetic
• rat plasma
• UPLC

In this study, a simple, sensitive, and robust analytical method based on ultra-performance liquid chromatography (UPLC) has been developed for the determination of acacetin in rat plasma using galangin as internal standard (IS). After sample preparation by a simple liquid-liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1 x 50 mm, 1.7 mu m particle size) and ultraviolet detection set at a wavelength of 333 nm. The method was linear over the concentration range 10-1500 ng/mL with a lower limit of quantification (LLOQ) of 10 ng/mL. Inter-and intra-day precision (RSD%) were all within 8.9% and the accuracy (RE%) was equal or lower than 4.8%. Recoveries of acacetin and IS were more than 80.2%. Stability studies showed that acacetin was stable under a variety of storage conditions. The method was successfully applied to a pharmacokinetic study involving intravenous administration of acacetin to rats.

• 出版日期2015
• 单位温州医科大学