Elucidation of the growth delimitation of Dunaliella tertiolecta under nitrogen stress by integrating transcriptome and peptidome analysis

作者：Shin HyeonSeok; Hong Seong Joo; Kim Hyojin; Yoo Chan; Lee Hookeun; Choi Hyung Kyoon; Lee Choul Gyun; Cho Byung Kwan^*

来源：Bioresource Technology, 2015, 194: 57-66.

DOI：10.1016/j.biortech.2015.07.002

• De novo transcriptome assembly
• Peptidome analysis
• Dunaliella tertiolecta
• Growth limitation
• Microalgae

Dunaliella tertiolecta LB 999 is an oleaginous microalgae species that produces large quantities of lipid and starch during nitrogen starvation; however, nitrogen starvation also limits the cell growth. In order to understand the underlying mechanisms of this phenomenon, the transcriptome and peptidome of D. tertiolecta LB 999 grown under different nitrogen and light conditions were analyzed. Integration of the de novo assembly of transcriptome sequencing reads with peptidome analysis revealed 13,861 protein-coding transcripts, including 33 transcripts whose expression patterns were significantly altered along with the growth phenotypes. Interestingly, 21 of these genes, which were highly enriched in the plastid region, were associated with chlorophyll synthesis and tetrahydrofolate-mediated C1 metabolism. Furthermore, intracellular glutamate levels are predicted to be the main factor that acts as a switch for the regulation of cell growth and carbon accumulation. These data provide the genetic information of D. tertiolecta for its future applications.

• 出版日期2015-10