Superoxide Biosensor Based on Superoxide Dismutase/Nano-ZnO

作者：Deng Zi Feng; Xu Wei; Yang Xiao^*

来源：Chemical Journal of Chinese Universities, 2009, 30(1): 37-41.

• ZnO
• Superoxide dismutase
• Superoxide anion
• Biosensor

A nanoporous ZnO film (nano-ZnO) Was prepared by physical vapor deposited method and its physical and chemical properties were studied by means of SEM, UV-Vis, XRD and electrochemical methods. The results show that nano-ZnO is a nanoporous film with a polycrystalline hexagonal wurtzite structure. The average diameter of the nanoparticles is about 5C-100 nm and its band gap is 3.37 eV. A new superoxide dismutase(SOD) modified nano-ZnO electrode(SOD/ZnO) Was prepared by dipping method. SOD Was modified stably in the nano-ZnO film confirmed by electrochemical impedance spectroscopy (EIS) and the direct electron transfer could he achieved confirmed by cyclic voltammetry (CV). Spectrum studies indicated that SOD kept Its bio-activities. A novel third-generation O(2)(-) biosensor With high selectivity, quick response time (4s), large linear range (anodic current response: 0.24-180 x 10(-6) mol/L; cathodic current response: 0.12-250 x 10(-6) mol/L) and good sensitivity (anodic: 2 x 10(-7) mol/L; cathodic: 1 x 10(-7) mol/L) was constructed and its analytical properties were studied.

• 出版日期2009-1-10
• 单位同济大学