The Development and Validation of Novel, Simple High-Performance Liquid Chromatographic Method with Refractive Index Detector for Quantification of Memantine Hydrochloride in Dissolution Samples

作者：Sawant Tukaram B^*; Wakchaure Vikas S; Rakibe Udyakumar K; Musmade Prashant B; Chaudhari Bhata R; Mane Dhananjay V

来源：Journal of Chromatographic Science, 2017, 55(6): 603-609.

DOI：10.1093/chromsci/bmx013

The present study was aimed to develop an analytical method for quantification of memantine (MEM) hydrochloride in dissolution samples using high-performance liquid chromatography with refractive index (RI) detector. The chromatographic separation was achieved on C18 (250 x 4.5 mm, 5 mu m) column using isocratic mobile phase comprises of buffer (pH 5.2): methanol (40: 60 v/v) pumped at a flow rate of 1.0mL/min. The column effluents were monitored using RI detector. The retention time of MEM was found to be similar to 6.5 +/- 0.3min. The developed chromatographic method was validated and found to be linear over the concentration range of 5.0-45.0 mu g/mL for MEM. Mean recovery of MEM was found to be 99.2 +/- 0.5% (w/w). The method was found to be simple, fast, precise and accurate, which can be utilized for the quantification of MEM in dissolution samples.

• 出版日期2017-7