科研之友
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摘要
The authors describe a colorimetric assay for the determination of organophosphorous pesticides (OPPs) based on enzyme-triggered deposition of silver nanoparticles on the surface of gold nanoparticles (AuNP). In this method, alkaline phosphatase (ALP) catalyzes the dephosphorylation of the substrate p-aminophenyl phosphate (p-APP) to form p-aminophenol (p-AP) which is capable of reducing Ag(I) ion to Ag metal which is spontaneously deposited on the surface of AuNP to form Au@Ag NPs. As a result, the color of the colloidal solution first changes from red to yellow, and then to gray with further increases in the thickness of the Ag shell. This can be detected visually or by spectrophotometry. OPPs act as inhibitors of ALP so that the dephosphorylation of p-APP is blocked and silver deposition on the AuNP is retarded or completely suppressed. As a result, the color change from red to yellow is less distinct. This finding forms the basis for the determination of OPPs. Under optimum conditions, the absorbance at 370 nm depends linearly on the logarithm of inhibitor concentration over the ranges from 0.05 to 500 mu gai...L-1 and from 0.1 to 500 mu gai...L-1, with detection limits of 0.025 mu gai...L-1 for methamidophos and 0.036 mu gai...L-1 for malathion (at an S/N ratio of 3). Both values are much lower than the maximum residue limits specified in the U.S. Department of Agriculture and European Union pesticide regulations. The validation and practicability of this method for the measurement of OPPs was demonstrated by analyzing (spiked) tap water and lake water.
