A Central Role for the WH2 Domain of Srv2/CAP in Recharging Actin Monomers to Drive Actin Turnover In Vitro and In Vivo

作者：Chaudhry Faisal; Little Kristin; Talarico Lou; Quintero Monzon Omar; Goode Bruce L^*

来源：Cytoskeleton, 2010, 67(2): 120-133.

DOI：10.1002/cm.20429

• actin
• yeast
• turnover
• Srv2/CAP
• ADF/cofilin
• profiling
• WH2 domain

Cellular processes propelled by actin polymerization require rapid disassembly of filaments, and then efficient recycling of ADF/cofilin-bound ADP-actin monomers back to an assembly-competent ATP-bound state. How monomer recharging is regulated in vivo is still not well understood, but recent work suggests the involvement of the ubiquitous actin-monomer binding protein Srv2/CAP. To better understand Srv2/CAP mechanism, we explored the contribution of its WH2 domain, the function of which has remained highly elusive. We found that the WH2 domain binds to actin monomers and, unlike most other WH2 domains, exhibits similar binding affinity for ATP-actin and ADP-actin (K-d similar to 1.5 mu M). Mutations in the WH2 domain that impair actin binding disrupt the ability of purified full-length Srv2/CAP to catalyze nucleotide exchange on ADF/cofilin-bound actin monomers and accelerate actin turnover in vitro. The same mutations impair Srv2/CAP function in vivo in regulating actin organization, cell growth, and cell morphogenesis. Thus, normal cell growth and organization depend on the ability of Srv2/CAP to recharge actin monomers, and the WH2 domain plays a central role in this process. Our data also reveal that while most isolated WH2 domains inhibit nucleotide exchange on actin, WH2 domains in the context of intact proteins can help promote nucleotide exchange.

• 出版日期2010-2