Altered Gut Microbiota Promotes Colitis-Associated Cancer in IL-1 Receptor-Associated Kinase M-Deficient Mice

作者：Klimesova Klara^*; Kverka Miloslav; Zakostelska Zuzana; Hudcovic Tomas; Hrncir Tomas; Stepankova Renata; Rossmann Pavel; Ridl Jakub; Kostovcik Martin; Mrazek Jakub; Kopecny Jan; Kobayashi Koichi S; Tlaskalova Hogenova Helena

来源：Inflammatory Bowel Diseases, 2013, 19(6): 1266-1277.

DOI：10.1097/MIB.0b013e318281330a

• cancer in IBD
• mucosal immunity
• colorectal cancer
• microbiota
• germ-free

Background:Microbial sensing by Toll-like receptors (TLR) and its negative regulation have an important role in the pathogenesis of inflammation-related cancer. In this study, we investigated the role of negative regulation of Toll-like receptors signaling and gut microbiota in the development of colitis-associated cancer in mouse model.Methods:Colitis-associated cancer was induced by azoxymethane and dextran sodium sulfate in wild-type and in interleukin-1 receptor-associated kinase M (IRAK-M)-deficient mice with or without antibiotic (ATB) treatment. Local cytokine production was analyzed by multiplex cytokine assay or enzyme-linked immunosorbent assay, and regulatory T cells were analyzed by flow cytometry. Changes in microbiota composition during tumorigenesis were analyzed by pyrosequencing, and -glucuronidase activity was measured in intestinal content by fluorescence assay.Results:ATB treatment of wild-type mice reduced the incidence and severity of tumors. Compared with nontreated mice, ATB-treated mice had significantly lower numbers of regulatory T cells in colon, altered gut microbiota composition, and decreased -glucuronidase activity. However, the -glucuronidase activity was not as low as in germ-free mice. IRAK-M-deficient mice not only developed invasive tumors, but ATB-induced decrease in -glucuronidase activity did not rescue them from severe carcinogenesis phenotype. Furthermore, IRAK-M-deficient mice had significantly increased levels of proinflammatory cytokines in the tumor tissue.Conclusions:We conclude that gut microbiota promotes tumorigenesis by increasing the exposure of gut epithelium to carcinogens and that IRAK-M-negative regulation is essential for colon cancer resistance even in conditions of altered microbiota. Therefore, gut microbiota and its metabolic activity could be potential targets for colitis-associated cancer therapy.

• 出版日期2013-5