Histological and Immunohistochemical Studies on the Furstenberg's Rosette in Cows

作者：Asti Resat Nuri; Kurtdede Nevin; Altunay Hikmet; Alabay Belma; Ozen Asuman; Bayraktaroglu Alev Gurol^*

来源：Kafkas Universitesi Veteriner Fakultesi Dergisi, 2011, 17(2): 223-228.

• Furstenberg's rosette
• FAE
• FALT
• Mammary gland
• Cow

The purpose of this study was to investigate the light and electron microscopic structure of Furstenberg's rosette region and to determine of uptake of macromolecules from follicle associated epithelium (FAE) of healthy cows in lactation period. In this study, 26 distal teat end taken from 10 animals in lactation period were used as material. Tissue samples were prepared for examination by light and transmission electron microscope. Indirect immunoperoxidase method was implemented to determine of cells containing IgA and IgG. Ferritin solution (25 mg/ml) was applied to distal teat end 1 h before slaughtered to show uptake of macromolecules in FAE. It was observed that Furstenberg's rosette region consists of lymphoid and nonlymphoid areas. Solitary and aggregate lymphoid follicles were seen in lymphoid areas. It was attracted attention that upper surfaces of these follicles were covered with FAE. IgA positive cells were seen in centrum germinativum of lymphoid follicles in the lymphoid region and subepithelial areas. High endothelial venules (HEV), which is characteristic feature of lympho-epithelial tissues, were observed at connective tissue which is around of the lymphoid follicles. In thin sections, the teat end tissue have been previously applied to ferritin, ferritin particles were detected in apical invaginations and pinocytotic vesicles of membranous cells (M cells). It was determined that there is Furstenbeg's rosette associated lymphoid tissue (FALT) in teat end and this region was generated spesific immune response against antigens after their uptaking and proccessing. Thus, we have reached conclusion that FALT may play a role mucosal immunity of teat end.

• 出版日期2011