Design of an antibacterial gelatin based on a covalent protein-protein coupling

作者：Lequeux Isabelle; Ducasse Emmanuel; Jouenne Thierry; Thebault Pascal^*

来源：Journal of Applied Polymer Science, 2015, 132(15): 41825.

DOI：10.1002/app.41825

• biomaterials
• biomedical applications
• biopolymers and renewable polymers

An antibacterial peptide (AMP), i.e., nisin, was covalently bound to gelatin through a proteinprotein coupling. Various reaction conditions were tested to study and optimize parameters of grafting e.g., orientation and density of AMP, which could impact the final antibacterial activity of the modified biopolymer. Modification was investigated by Fourier transform infrared (FT-IR) spectroscopy and zeta potential. The antibacterial activity of the nisin-enriched gelatin was evaluated against two staphylococci bacterial strains, i.e., Staphylococus epidermidis and Staphylococcus aureus. A higher activity was found for gelatin modified at pH= 7.4 revealing an influence of the nisin orientation on the protein antibacterial property.

• 出版日期2015-4-15