Mechanistic insights into nanotoxicity determined by synchrotron radiation-based Fourier-transform infrared imaging and multivariate analysis

作者：Riding Matthew J; Trevisan Julio; Hirschmugl Carol J; Jones Kevin C; Semple Kirk T; Martin Francis L^*

来源：Environment International, 2012, 50: 56-65.

DOI：10.1016/j.envint.2012.09.009

• Carbon nanoparticles
• Multivariate analysis
• Nanotoxicity
• Spectroscopy
• Synchrotron radiation

Our ability to identify the mechanisms by which carbon-based nanomaterials (CBNs) exert toxicity in cells is constrained by the lack of standardized methodologies to assay endpoint effects. Herein we describe a method of mechanistically identifying the effects of various CBN types in both prokaryotic and eukaryotic cells using multi-beam synchrotron radiation-based Fourier-transform infrared imaging (SR-FTIRI) at diffraction-limited resolution. This technique overcomes many of the inherent difficulties of assaying nanotoxicity and demonstrates exceptional sensitivity in identifying the effects of CBNs in cells at environmentally-relevant concentrations. We identify key mechanisms of nanotoxicity as the alteration of Amide and lipid biomolecules, but propose more specific bioactivity of CBNs occurs as a result of specific interactions between CBN structural conformation and cellular characteristics.

• 出版日期2012-12-1