Application of dual F-19 and iron cellular MRI agents to track the infiltration of immune cells to the site of a rejected stem cell transplant

作者：Gaudet Jeffrey M; Hamilton Amanda M; Chen Yuanxin; Fox Matthew S; Foster Paula J^*

来源：Magnetic Resonance in Medicine, 2017, 78(2): 713-720.

DOI：10.1002/mrm.26400

• F-19-MRI
• fluorine-19
• cellular MRI
• mesenchymal stem cells
• transplant rejection

PurposeCellular MRI) was used to detect implanted human mesenchymal stem cells (hMSCs) and the resulting macrophage infiltration that occurs in response to xenotransplantation.
MethodsHuman mesenchymal stem cells were prelabeled with a fluorine-19 (F-19) agent prior to implantation, allowing for their visualization and quantification over time. Following implantation of 1x10(6) F-19-labeled hMSCs into the mouse hind limb, longitudinal imaging was performed to monitor the stem cell graft. Macrophages were labeled in situ by the intravenous administration of an ultrasmall superparamagentic iron oxide (USPIO), allowing for tracking of the inflammatory response.
ResultsQuantification of F-19 MRI on day 0 agreed with the implanted number of cells, and F-19 signal decreased over time. By day 14, only 22%11% of the original F-19 signal remained. In a second group, USPIO were administered intravenously after implantation of F-19-labeled hMSCs. When imaged on day 2, a significant decrease in F-19 signal was observed compared to the first group alongside a large signal void region in the corresponding proton images. Immunohistochemistry confirmed the presence of iron-labeled macrophages in the stem cell tract.
ConclusionA dual-labeling technique was used to noninvasively track two distinct cell populations simultaneously. This information could be used to provide additional insight into the cause of graft failure. Magn Reson Med 78:713-720, 2017.

• 出版日期2017-8