Analysis of alkaloids in Semen nelumbinis by accelerated solvent extraction-high performance liquid chromatography-diode array detection-electrospray ioninztion-time of flight-mass spectrometry

作者：Li Wen-Long; Chen Jun-Hui; Yin Yue-Fen; Wang Xiao-Ru^*; Lee, Frank S. C.

来源：Chinese Journal of Analytical Chemistry, 2008, 36(1): 79-82.

• Semen nelumbinis
• alkaloids
• time of flight mass spectrometry
• acceleratied solvent extraction

An accelerated solvent extraction-high performance liquid chromatography-diode array detection-electrospray ioninztion-time of flight-mass spectrometry (ASE-HPLC-DAD-ESI-TOF/MS) analysis method was developed for the analysis of lkaloids in Semen nelumbinis. The samples were extracted using ASE for 10 min at 100 T and 9646 kPa, and then analyzed by HPLC-DAD-ESI-TOF/MS. HPLC was carried out, using an Agilent Eclipse XDB-C-18 column(250 min x 4.6 min x 5 mu m) and acetonitrile-0.1% triethylamine (adjusted pH = 8. 0 with ammonia solution) as mobile phase ( using gradient elution) with flow rate 1.0 mL/min and detected at 282 nm. The ESI-TOF/MS was set at the positive ion mode; capillary voltage:4500 V; pressure of spray: 310.05 kPa; fiow velocity of dry gas (N-2) : 10.0 L/min; temperature of dry gas: 350 degrees C; fragmentor voltage: 150 V. Comparing the spectrogram and mass spectrum of the chromatogram peak with the references value, six compounds were identified as pronucifefine, lotusine, nuciferine, liensinine, isolisensinine, and neferine.

• 出版日期2008-1
• 单位国家海洋局第一海洋研究所; 中国海洋大学; 厦门大学