A Calcium-Cross-Linked Hydrogel Based on Alginate-Modified Atelocollagen Functions as a Scaffold Material

作者：Kamimura Wataru; Hattori Rieko; Koyama Hiroyuki^*; Miyata Tetsuro; Takato Tsuyoshi

来源：Journal of Biomaterials Science, Polymer Edition, 2012, 23(5): 609-628.

DOI：10.1163/092050611X555678

• Tissue engineering
• regenerative tissue
• scaffold material
• hydrogel
• alginate
• collagen
• basic fibroblast growth factor

We have developed a scaffold material consisting of a covalently-bonded structure of alginate and atelocollagen (AtCol). Addition of calcium ions caused the material to form a hydrogel (alginate-modified AtCol gel). The condition of the alginate-modified AtCol gel could be controlled by the feed ratio of alginate and the activating reagent. Measurement of temporal stability in culture medium suggested that covalent bonding between alginate and AtCol might contribute to the structural stability of the alginate-modified AtCol gel. Culture with endothelial cells indicated that cell adhesiveness on the alginate-modified AtCol gel was similar to that on native collagen. Collagenase digestion revealed that the alginate-modified AtCol gel had considerable ability to retain basic fibroblast growth factor. Additionally, active cell migration into alginate-modified AtCol was detected by in vitro assay using endothelial cells. These findings indicate that this gel material can be expected to function as a scaffold for inducing vascular in-growth.

• 出版日期2012