Application of arteether-loaded polyurethane nanomicelles to induce immune response in breast cancer model

作者：Jabbarzadegan Mona; Rajayi Hajar; Jahromi Mirza Ali Mofazzal; Yeganeh Hamid; Yousefi Mehdi; Hassan Zuhair Muhammad; Majidi Jafar^*

来源：Artificial Cells, Nanomedicine and Biotechnology, 2017, 45(4): 808-816.

DOI：10.1080/21691401.2016.1178131

• Cancer therapy
• immunostimulator
• nanocarrier
• 4T1

To concentrate a potent anticancer drug (Arteether) in tumor microenvironment, we encapsulated it in biodegradable and pH sensitive polyurethane (PU) nanomicelles (NMs). The nanocomplex was characterized by Fourier transform infrared (FTIR), dynamic light scattering (DLS). The loading capacity and release profile in pH of 5.4 and 7.4 were considered. The cytotoxicity effect was evaluated in vitro and in vivo settings. The level of IFN- and IL-4 cytokines of mice splenocytes were assessed by enzyme-linked immunosorbent assay (ELISA). The nanocomplex showed negative zeta charge of -26.2mV, size of 42.30nm and high loading capacity (92%). Release profile showed a faster rate of drug liberation at pH 5.4 as compared to that of pH 7.4. It indicated significant inhibitory effect on the growth of 4T1 cell line and increased IFN- level.

• 出版日期2017-6