Avoiding cross hybridization by choosing nonredundant targets on cDNA arrays

作者：Nielsen HB^*; Knudsen S

来源：Bioinformatics, 2002, 18(2): 321-322.

DOI：10.1093/bioinformatics/18.2.321

PROBEWIZ designs PCR primers for amplifying probes for cDNA arrays. The probes are designed to have minimal homology to other expressed sequences from a given organism. The primer selection is based on user-defined penalties for homology, primer quality, and proximity to the 3' end.

• 出版日期2002-2