Monitoring Proteins Using In Vivo Near-Infrared Time-Domain Optical Imaging after 2-O-Hexyldiglycerol-Mediated Transfer to the Brain

作者：Huelper Petra; Dullin Christian; Kugler Wilfried; Lakomek Max; Erdlenbruch Bernhard

来源：Molecular Imaging and Biology, 2011, 13(2): 275-283.

DOI：10.1007/s11307-010-0348-3

• BBB opening
• 2-O-Hexyldiglycerol
• Anti TGF-beta antibody 1D11
• Alexa Fluor 680
• Cy5.5
• Time-domain optical imaging

The aim of the present study was to gain insight into the penetration, biodistribution, and fate of globulins in the brain after 2-O-hexyldiglycerol-induced blood-brain barrier opening.
The spatial distribution of fluorescence probes was investigated after blood-brain barrier opening with intracarotid 2-O-hexyldiglycerol injection. Fluorescence intensity was visualized by microscopy (mice and rats) and by in vivo time-domain optical imaging.
There was an increased 2-O-hexyldiglycerol-mediated transfer of fluorescence-labeled globulins into the ipsilateral hemisphere. Sequential in vivo measurements revealed that the increase in protein concentration lasted at least 96 h after administration. Ex vivo detection of tissue fluorescence confirmed the results obtained in vivo.
Globulins enter the healthy brain in conjunction with 2-O-hexyldiglycerol. Sequential in vivo near-infrared fluorescence measurements enable the visualization of the spatial distribution of antibodies in the brain of living small animals.

• 出版日期2011-4