A validated GC-MS method for the determination of Delta(9)-tetrahydrocannabinol and 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid in bile samples

作者：Papoutsis Ioannis; Nikolaou Panagiota^*; Dona Artemisia; Pistos Constantinos; Stefanidou Maria; Spiliopoulou Chara; Athanaselis Sotirios

来源：Forensic Toxicology, 2012, 30(1): 51-58.

DOI：10.1007/s11419-011-0126-1

• Delta(9)-Tetrahydrocannabinol
• 11-Nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid
• Bile
• GC-MS

We present a validated gas chromatography-mass spectrometry method for the determination of Delta(9)-tetrahydrocannabinol and 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid in bile samples. The method includes protein precipitation with acetonitrile after enzymatic hydrolysis, and solid-phase extraction followed by silylation using N,O-bis(trimethylsilyl)trifluoroacetamide with 1% trimethylchlorosilane. The limit of detection was 0.30 ng/ml and the limit of quantitation was 1.00 ng/ml. The calibration curves were linear within the dynamic range of 1.00-500 ng/ml (R (2) a parts per thousand yen 0.993) and the absolute recovery for both analytes was higher than 87.5%. Accuracy and precision were less than 8.8% and 8.2%, respectively. The developed method was applied for the analysis of bile samples obtained from 21 forensic cases.

• 出版日期2012-1