Arabidopsis G-protein interactome reveals connections to cell wall carbohydrates and morphogenesis

作者：Klopffleisch Karsten; Nguyen Phan; Augustin Kelsey; Bayne Robert S; Booker Katherine S; Botella Jose R; Carpita Nicholas C; Carr Tyrell; Chen Jin Gui; Cooke Thomas Ryan; Frick Cheng Arwen; Friedman Erin J; Fulk Brandon; Hahn Michael G; Jiang Kun; Jorda Lucia; Kruppe Lydia; Liu Chenggang; Lorek Justine; McCann Maureen C; Molina Antonio; Moriyama Etsuko N; Mukhtar M Shahid; Mudgil Yashwanti; Pattathil Sivakumar; Schwarz John; Seta Steven; Tan Matthew

来源：Molecular Systems Biology, 2011, 7(1): 532.

DOI：10.1038/msb.2011.66

• AGB1
• Arabidopsis
• GPA1
• heterotrimeric G-proteins
• RGS1

The heterotrimeric G-protein complex is minimally composed of G alpha, G beta, and G gamma subunits. In the classic scenario, the G-protein complex is the nexus in signaling from the plasma membrane, where the heterotrimeric G-protein associates with heptahelical G-protein-coupled receptors (GPCRs), to cytoplasmic target proteins called effectors. Although a number of effectors are known in metazoans and fungi, none of these are predicted to exist in their canonical forms in plants. To identify ab initio plant G-protein effectors and scaffold proteins, we screened a set of proteins from the G-protein complex using two-hybrid complementation in yeast. After deep and exhaustive interrogation, we detected 544 interactions between 434 proteins, of which 68 highly interconnected proteins form the core G-protein interactome. Within this core, over half of the interactions comprising two-thirds of the nodes were retested and validated as genuine in planta. Co-expression analysis in combination with phenotyping of loss-of-function mutations in a set of core interactome genes revealed a novel role for G-proteins in regulating cell wall modification. Molecular Systems Biology 7: 532; published online 27 September 2011; doi:10.1038/msb.2011.66

• 出版日期2011-9