A General Method To Increase Stokes Shift by Introducing Alternating Vibronic Structures

作者：Ren, Tian-Bing; Xu, Wang; Zhang, Wei; Zhang, Xing-Xing; Wang, Zhi-Yao; Xiang, Zhen; Yuan, Lin^*; Zhang, Xiao-Bing

来源：Journal of the American Chemical Society, 2018, 140(24): 7716-7722.

DOI：10.1021/jacs.8b04404

• NEAR-INFRARED FLUORESCENCE
• HEPTAMETHINE CYANINE DYES
• EMISSION WAVELENGTH
• SI-RHODAMINE
• LIVE-CELL
• BRIGHT
• PROBE
• FLUOROPHORES
• ENHANCEMENT
• UNIQUE

Fluorescent dyes have enabled much progress in the broad range of biomedical fields. However, many commercially available dyes suffer from small Stokes shifts, resulting in poor signal-to-noise ratio and self-quenching on current microscope configurations. In this work, we have developed a general method to significantly increase the Stokes shifts of common fluorophores. By simply appending a 1,4-diethyl-decahydro-quinoxaline (DOJ moiety onto the conjugated structure, we introduced a vibronic backbone that could facilely expand the Stokes shifts, emission wavelength, and photostability of 11 different fluorophores by more than 3-fold. This generalizable method could significantly improve the imaging efficiency of commercial fluorophores. As a demonstration, we showed that the DQ derivative of hemicyanine generated 5-fold signal in mouse models over indocyanine green. Furthermore, the DQ-modified fluorophores could pair with their parent molecules to conduct one-excitation, multiple emission imaging, allowing us to study the cell behavior more robustly. This approach shows promise in generating dyes suitable for super-resolution microscopy and second window near-infrared imaging.

• 出版日期2018-6-20
• 单位化学生物传感与计量学国家重点实验室; 湖南大学