A Simple Self-Calibrating Method To Measure the Height of Fluorescent Molecules and Beads at Nanoscale Resolution

作者：Kerssemakers Jacob W J; Blosser Timothy R; Dekker Cees^*

来源：Nano Letters, 2014, 14(8): 4469-4475.

DOI：10.1021/nl501434v

• Height determination
• fluorescence
• total internal reflection fluorescence
• nanoscale resolution

We describe a simple self-calibrating technique, incident-beam interference sweeping, for measuring the height of fluorescent labels. Using a tilted back-reflecting mirror and a scanning laser beam, a modulated fluorescence emission allows height determination of a label from a surface with a resolution of similar to 3 nm. In addition, we show that the absolute distance of a label from the top-mounted mirror can be determined with a resolution of a few tens of nanometers over a micrometer range.

• 出版日期2014-8