A force calibration standard for magnetic tweezers

作者：Yu Zhongbo; Dulin David; Cnossen Jelmer; Kober Mariana; van Oene Maarten M; Ordu Orkide; Berghuis Bojk A; Hensgens Toivo; Lipfert Jan; Dekker Nynke H^*

来源：Review of Scientific Instruments, 2014, 85(12): 123114.

DOI：10.1063/1.4904148

To study the behavior of biological macromolecules and enzymatic reactions under force, advances in single-molecule force spectroscopy have proven instrumental. Magnetic tweezers form one of the most powerful of these techniques, due to their overall simplicity, non-invasive character, potential for high throughput measurements, and large force range. Drawbacks of magnetic tweezers, however, are that accurate determination of the applied forces can be challenging for short biomolecules at high forces and very time-consuming for long tethers at low forces below similar to 1 piconewton. Here, we address these drawbacks by presenting a calibration standard for magnetic tweezers consisting of measured forces for four magnet configurations. Each such configuration is calibrated for two commonly employed commercially available magnetic microspheres. We calculate forces in both time and spectral domains by analyzing bead fluctuations. The resulting calibration curves, validated through the use of different algorithms that yield close agreement in their determination of the applied forces, span a range from 100 piconewtons down to tens of femtonewtons. These generalized force calibrations will serve as a convenient resource for magnetic tweezers users and diminish variations between different experimental configurations or laboratories.

• 出版日期2014-12